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STAT1 (Phospho-Ser727) Rabbit mAb#14197

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Product Detail

Product NameSTAT1 (Phospho-Ser727) Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC

Species ReactivityHuman Mouse Rat

SpecificityPhospho-STAT1 (S727) Antibody detects endogenous levels of Phospho-STAT1 (S727)

Immunogen DescA synthesized peptide derived from human Phospho-STAT1 (S727)

ConjugateUnconjugated

Other NamesSignal transducer and activator of transcription 1; Transcription factor ISGF-3 components p91/p84; STAT1; CANDF7; ISGF3;

Accession NoUniprot:P42224

Uniprot P42224

Calculated MWPredicted band size: 87 kDa

Sdspage MWObserved band size: 87 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes:STAT1 (Phospho-Ser727) Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with etoposide whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 87 kDa
Observed band size: 87 kDa

Exposure time: 5 seconds
All lanes:STAT1 (Phospho-Ser727) Rabbit mAb at 1/1k dilution

Lane 1 : Mouse heart lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat heart lysates
Lane 4 : Rat brain lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 87 kDa
Observed band size: 87 kDa

Exposure time: 8 seconds
All lanes : STAT1 (Phospho-Ser727) Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : STAT1 (Phospho-Ser727) knockdown HAP1 cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for STAT1 (Phospho-Ser727) using 14197 at 1/100 dilution in immunohistochemical analysis.

If you have published an article using product 14197, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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