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Location： Home > Phospho Antibodies > BTK (Phospho-Tyr551) Rabbit mAb

BTK (Phospho-Tyr551) Rabbit mAb#14198

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Product Detail

Product NameBTK (Phospho-Tyr551) Rabbit mAb

Clone No.SR2188

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityHuman

SpecificityPhospho-BTK (Y551) Antibody detects endogenous levels of Phospho-BTK (Y551)

Immunogen DescA synthesized peptide derived from human Phospho-BTK (Y551)

ConjugateUnconjugated

Other NamesBTK, AGMX1, AT, ATK, XLA, PSCTK1, B-cell progenitor kinase, BPK, Bruton tyrosine kinase, Tyrosine-protein kinase BTK, IMD1;

Accession NoUniprot:Q06187

Uniprot Q06187

Calculated MWPredicted band size: 76 kDa

Sdspage MWObserved band size: 74 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
All lanes : BTK (Phospho-Tyr551) Rabbit mAb at 1/1k dilution
Lane 1 :Ramos cell lysate
Lane 2 : Ramos cell lysate treated with Pervanadate.
lysate/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 74 kDa
Exposure time: 3 seconds
All lanes : BTK (Phospho-Tyr551) Rabbit mAb at 1/1k dilution
Lane 1 :K562 lysate
Lane 2 :THP-1 lysate
Lane 3 :Raji lysate
Lane 4 :MOLT-4 lysate
lysate/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 74 kDa
Exposure time: 9 seconds

If you have published an article using product 14198, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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