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Smad5 (Phospho-Ser463/Ser465) Rabbit mAb#14208

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Product Detail

Product NameSmad5 (Phospho-Ser463/Ser465) Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB ICC/IF

Species ReactivityHuman

SpecificityPhospho-Smad5 (S463/S465) Antibody detects endogenous levels of Phospho-Smad5 (S463/S465)

Immunogen DescA synthesized peptide derived from human Smad5

ConjugateUnconjugated

Other NamesMothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5; SMAD5; MADH5;

Accession NoUniprot:Q99717

Uniprot Q99717

Calculated MW52kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB:1:500~1:1000
ICC
1:50~1:200
Western blot analysis of Phospho-Smad5 (S463/S465) expression in (1) HeLa cell lysate; (2) HeLa cell treated with BMP-4 lysate.

If you have published an article using product 14208, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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