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Location： Home > KD/KO Validation > Smad2 (Phospho-Ser250) Rabbit mAb

Smad2 (Phospho-Ser250) Rabbit mAb#14211

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Product NameSmad2 (Phospho-Ser250) Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB FC

Species ReactivityHuman Mouse Rat

SpecificityPhospho-Smad2 (S250) Antibody detects endogenous levels of Phospho-Smad2 (S250)

Immunogen DescA synthesized peptide derived from human Smad2

ConjugateUnconjugated

Other NamesJV18-1, MADH2, MADR2, Mad-related protein 2, Mothers against DPP homolog 2, Mothers against decapentaplegic homolog 2, Smad 2;

Accession NoUniprot:Q15796

Uniprot Q15796

Calculated MW58kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details

WB:1:1000~1:2000
FC:1:20

All lanes: Smad2 (Phospho-Ser250) Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with alkaline phosphatase whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 52 kDa
Observed band size: 58 kDa

Exposure time: 7 seconds
All lanes: Smad2 (Phospho-Ser250) Rabbit mAb at 1/1k dilution

Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 52 kDa
Observed band size: 58 kDa

Exposure time: 10 seconds
All lanes :Smad2 (Phospho-Ser250) Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type HT-1080 cell lysate
Lane 2 : Smad2 (Phospho-Ser250) knockdown HT-1080 cell lysate

Lysates/proteins at 20 µg per lane.

If you have published an article using product 14211, please notify us so that we can cite your literature.

et al,Characterization of miR-34a-Induced Epithelial–Mesenchymal Transition in Non-Small Lung Cancer Cells Focusing on p53 , (2021),
PMID: 34944497

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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