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Location： Home > Phospho Antibodies > AMPK alpha 2 (Phospho-Ser491) Rabbit mAb

AMPK alpha 2 (Phospho-Ser491) Rabbit mAb#14221

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Product NameAMPK alpha 2 (Phospho-Ser491) Rabbit mAb

Clone No.SR2078

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityHuman Mouse Rat

SpecificityPhospho-AMPK alpha 2 (S491) Antibody detects endogenous levels of Phospho-AMPK alpha 2 (S491)

Immunogen DescA synthesized peptide derived from human AMPK alpha 2

ConjugateUnconjugated

Other Names5'-AMP-activated protein kinase catalytic subunit alpha-2; ACACA kinase; Acetyl-CoA carboxylase kinase; AMPK alpha 2 chain; AMPK subunit alpha-2; AMPK2; AMPKalpha2; PRKAA2;

Accession NoUniprot:P54646

Uniprot P54646

Calculated MWPredicted band size: 62 kDa

Sdspage MWObserved band size: 62 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
All lanes : AMPK alpha 2 (Phospho-Ser491) Rabbit mAb at 1/1k dilution
Lane 1 :293T treated with Lambda phosphatase for 30min cell lysate
Lane 2 : 293T cell lysate
lysate/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 5 seconds
All lanes : AMPK alpha 2 (Phospho-Ser491) Rabbit mAb at 1/1k dilution
Lane 1 :Rat liver lysate
Lane 2 : U-87 MG lysate
lysate/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 6 seconds

If you have published an article using product 14221, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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