Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > KD/KO Validation > AKT1 (Phospho-Ser129) Rabbit mAb

AKT1 (Phospho-Ser129) Rabbit mAb#14222

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NameAKT1 (Phospho-Ser129) Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityHuman Mouse Rat

SpecificityPhospho-AKT1 (S129) Antibody detects endogenous levels of Phospho-AKT1 (S129)

Immunogen DescA synthesized peptide derived from human AKT1

ConjugateUnconjugated

Other NamesAKT 1; PKB; PKB-ALPHA; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; RAC Alpha; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha;

Accession NoUniprot:P31749

Uniprot P31749

Calculated MW60kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB:1:1000~1:2000
All lanes: AKT1 (Phospho-Ser129) Rabbit mAb at 1/1k dilution

Lane 1 : MCF7 treated with alkaline phosphatase whole cell lysates
Lane 2 : MCF7 whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 56 kDa
Observed band size: 60 kDa

Exposure time: 5 seconds
All lanes: AKT1 (Phospho-Ser129) Rabbit mAb at 1/1k dilution

Lane 1 : Mouse heart lysates
Lane 2 : Mouse lung lysates
Lane 3 : Rat heart lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 56 kDa
Observed band size: 60 kDa

Exposure time: 7 seconds
All lanes:AKT1 (Phospho-Ser129)Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : AKT1 (Phospho-Ser129) knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.

If you have published an article using product 14222, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors