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Location： Home > KD/KO Validation > STAT3 (Phospho-Tyr705) Rabbit mAb

STAT3 (Phospho-Tyr705) Rabbit mAb#14235

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Product NameSTAT3 (Phospho-Tyr705) Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB IHC ICC/IF IP

Species ReactivityHuman Mouse Rat

SpecificityPhospho-STAT3 (Y705) Antibody detects endogenous levels of total Phospho-STAT3 (Y705)

Immunogen DescA synthesized peptide derived from human STAT3

ConjugateUnconjugated

Other NamesAPRF; Stat3; HIES; Acute-phase response factor;

Accession NoUniprot:P40763

Uniprot P40763

Calculated MW88kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details

WB:1:5000~1:10000
IHC:1:50~1:100
ICC/IF:1:50~1:000
IP:1:30

All lanes: STAT3 (Phospho-Tyr705) Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with 100ng/ml IFNa for 5min whole cell lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 88 kDa
Observed band size: 88 kDa

Exposure time: 7 seconds
All lanes: STAT3 (Phospho-Tyr705) Rabbit mAb at 1/1k dilution

Lane 1 : Mouse lung lysates
Lane 2 : Mouse kidney lysates
Lane 3 : Rat lung lysates
Lane 2 : Rat skin lysates
Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 88 kDa
Observed band size: 88 kDa

Exposure time: 11 seconds
All lanes:STAT3 (Phospho-Tyr705) Rabbit mAb at 1/1k dilution

Lane 1 : Wild-type Hela cell lysate
Lane 2 : STAT3 (Phospho-Tyr705) knockdown Hela cell lysate

Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human pancreas tissue stained for STAT3 (Phospho-Tyr705) using 14235 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence STAT3 (Phospho-Tyr705) antibody (14235)

ICC/IF staining of STAT3 (Phospho-Tyr705) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14235 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

If you have published an article using product 14235, please notify us so that we can cite your literature.

et al,AHR Agonist ITE Boosted PD1 Antibody’s Effects by Inhibiting Myeloid-Derived Cells Suppressive Cells in an Orthotopic Mouse Glioma Model , (2025),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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