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Location： Home > KD/KO Validation > eIF4B (Phospho-Ser406) Rabbit mAb

eIF4B (Phospho-Ser406) Rabbit mAb#14278

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Product NameeIF4B (Phospho-Ser406) Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB;IHC;IF

Species ReactivityHuman;Mouse;Rat

SpecificityPhospho-eIF4B (S406) Antibody detects endogenous levels of total Phospho-eIF4B (S406)

Immunogen DescA synthesized peptide derived from human Phospho-eIF4B (S406)

ConjugateUnconjugated

Other NamesEIF4B;

Accession NoUniprot:P23588

Uniprot P23588

Calculated MW69 kDa

Sdspage MW75 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details

WB:1:500~1:2000
IHC:1:50~1:200

All lanes : Phospho-eIF4B (S406) Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : 293T whole cell lysates
Lane 3 : K562 whole cell lysates
Lane 4 : HepG2 whole cell lysates
Lane 5 : 3T3 whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 69 kDa
Observed band size: 75 kDa

Exposure time: 3 seconds
All lanes : Phospho-eIF4B (S406) Rabbit mAb at 1/1k dilution

Lane 1 : Mouse Liver lysates
Lane 2 : Rat Liver lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 69 kDa
Observed band size: 75 kDa

Exposure time: 3 seconds
All lanes : Phospho-eIF4B (S406) Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with 100nM Insulin for 20min whole cell lysates
Lane 3 : 293T whole cell lysates
Lane 4 : 293T treated with Lambda Protein Phosphatase for 30min whole cell lysates
Lane 5 : 293T treated with 100nM Insulin for 20min whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 69 kDa
Observed band size: 75 kDa

Exposure time: 6 seconds
Formalin-fixed, paraffin-embedded mouse lung tissue stained for Phospho-eIF4B (S406) using 14278 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded rat lung tissue stained for Phospho-eIF4B (S406) using 14278 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence p-eIF4B (S406) antibody (14278)

14278 staining p-eIF4B (S406) in the HeLa cell line by ICC/IF. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14278 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
The negative control is shown in bottom right hand panel - for the negative control, purified 14278 was used at a dilution of 1/100 followed by Alexa Fluor® 647 goat anti- rabbit antibody at a dilution of 1/500.
Nuclei were counterstained with DAPI.

If you have published an article using product 14278, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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