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Vimentin (Phospho-S39) Rabbit mAb#14301

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Product Detail

Product NameVimentin (Phospho-S39) Rabbit mAb

Clone No.SR1953

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityHuman Mouse Rat

SpecificityPhospho-Vimentin (S39) Antibody detects endogenous levels of total Phospho-Vimentin (S39)

Immunogen DescA synthesized peptide derived from human Phospho-Vimentin (S39)

ConjugateUnconjugated

Other NamesVIM; VIME; Vimentin;

Accession NoP08670

Uniprot P08670

Calculated MWPredicted band size: 54 kDa

Sdspage MWObserved band size: 54 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

Application Details
WB: 1:500-1:2000
All lanes : Vimentin (Phospho-S39) Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with 100nM Calyculin A for 30min whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 2 seconds

  Phospho-Vimentin (S39) Antibody detects endogenous levels of total Phospho-Vimentin (S39)

If you have published an article using product 14301, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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