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Recombinant Rabbit Monoclonal Antibodies > Akt (Phospho-Thr308) Rabbit mAb
Akt (Phospho-Thr308) Rabbit mAb#14308
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All lanes : Akt (Phospho-Thr308) Rabbit mAb at 1/1k dilutionLane 1 : JK whole cell lysatesLane 2 : 293T whole cell lysatesLane 3 : 3T3 whole cell lysatesLane 4 : 3T3 treated with 100nM insulin for 20min whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 56 kDa
Observed band size: 60 kDaExposure time: 7 seconds
Immunocytochemistry/Immunofluorescence Akt (Phospho-Thr308) antibody (14308)
ICC/IF staining of Akt (Phospho-Thr308) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14308 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
The negative control is shown in bottom right hand panel - for the negative control. Nuclei were counterstained with DAPI.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
