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Location： Home > Recombinant Rabbit Monoclonal Antibodies > IGF1R (Phospho-Tyr1135/1136)/Insulin Receptor β (Phospho-Tyr1150/1151) Rabbit mAb

IGF1R (Phospho-Tyr1135/1136)/Insulin Receptor β (Phospho-Tyr1150/1151) Rabbit mAb#14314

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Product NameIGF1R (Phospho-Tyr1135/1136)/Insulin Receptor β (Phospho-Tyr1150/1151) Rabbit mAb

Clone No.SR4355

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProtein A

ApplicationsWB

Species ReactivityHuman;Mouse;Rat

SpecificityAntibody detects endogenous levels of IGF-I receptor and insulin receptor only when phosphorylated at tyrosine 1135/1136 or tyrosine 1150/1151, respectively.

Target NameIGF IR

Other NamesIGF1R;Insulin-like growth factor 1 receptor;Insulin-like growth factor I receptor;IGF-I receptor;CD antigen CD221;INSR;Insulin receptor;IR;CD antigen CD220

Accession NoP06213;P08069

Uniprot P06213;P08069

Calculated MWPredicted band size: 156 kDa

Sdspage MWObserved band size: 95 kDa

FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

StorageStore at -20°C /1 year

Application Details
WB: 1:500-1:2000
All lanes : IGF1R (Phospho-Tyr1135/1136)/Insulin Receptor β (Phospho-Tyr1150/1151) Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with 100nM insulin for 20min whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 156 kDa
Observed band size: 95 kDa
Exposure time: 10 seconds
All lanes : IGF1R (Phospho-Tyr1135/1136)/Insulin Receptor β (Phospho-Tyr1150/1151) Rabbit mAb at 1/1k dilution
Lane 1 : 3T3 whole cell lysates
Lane 2 : 3T3 treated with serum for 30min and 100nM IGF-1 for 2min whole cell lysates
Lane 3 : C6 whole cell lysates
Lane 4 : C6 treated with serum for 30min and 100nM IGF-1 for 2min whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 156 kDa
Observed band size: 95 kDa
Exposure time: 7 seconds

If you have published an article using product 14314, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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