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Location： Home > Recombinant Rabbit Monoclonal Antibodies > Syk (Phospho-Tyr525/526) Rabbit mAb

Syk (Phospho-Tyr525/526) Rabbit mAb#14325

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Product Detail

Product NameSyk (Phospho-Tyr525/526) Rabbit mAb

Clone No.SR4365

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProtein A

ApplicationsWB

Species ReactivityHuman;Mouse;Rat

SpecificityAntibody detects endogenous levels of syk protein only when phosphorylated at Tyr525/526 of human Syk or Tyr519/520 of mouse Syk.

Target NameSyk

Other NamesSYK;Tyrosine-protein kinase SYK;Spleen tyrosine kinase;p72-Syk

Accession NoP43405

Uniprot P43405

Calculated MWPredicted band size: 72 kDa

Sdspage MWObserved band size: 72 kDa

FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

StorageStore at -20°C /1 year

Application Details
WB: 1:500-1:2000
All lanes : Syk (Phospho-Tyr525/526) Rabbit mAb at 1/1k dilution
Lane 1 : Ramos whole cell lysates
Lane 2 : Ramos treated with 12 ug/ml anti-IgM for 2 minutes whole cell lysates
Lane 3 : C6 whole cell lysates
Lane 4 : C6 treated with 10 nM Na3VO4 for 1 h whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDa

Exposure time: 5 seconds

If you have published an article using product 14325, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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