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Recombinant Rabbit Monoclonal Antibodies > p70 S6 Kinase (Phospho-Thr389) Rabbit mAb
p70 S6 Kinase (Phospho-Thr389) Rabbit mAb#14336
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All lanes : p70 S6 Kinase (Phospho-Thr389) Rabbit mAb at 1/1k dilutionLane 1 : C6 whole cell lysatesLane 2 : C6 was starved with serum for 24 hours and treated with 100ng/mL IGF-1 for 20 minutes whole cell lysatesLane 3 : 3T3 whole cell lysatesLane 4 : 3T3 was starved with serum for 24 hours and treated with 100ng/mL IGF-1 for 20 minutes whole cell lysatesLane 5 : Hela whole cell lysatesLane 6 : Hela was starved with serum for 24 hours and treated with 100ng/mL IGF-1 for 20 minutes whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 59 kDa Observed band size: 70 kDaExposure time: 10 seconds
Formalin-fixed, paraffin-embedded human liver tissue stained for p70 S6 Kinase (Phospho-Thr389) using 14336 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/Immunofluorescence p70 S6 Kinase (Phospho-Thr389) antibody (14336)
ICC/IF staining of p70 S6 Kinase (Phospho-Thr389) in 293T cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14336 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
The negative control is shown in bottom right hand panel - for the negative control. Nuclei were counterstained with DAPI.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
