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Location： Home > Polyclonal Antibodies > PDK1(Ab-241) Antibody

PDK1(Ab-241) Antibody#21005

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Product Detail

Product NamePDK1(Ab-241) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total PDK1 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.239~243 (A-N-S-F-V) derived from Human PDK1.

Target NamePDK1

ConjugateUnconjugated

Other NamesPDPK1, PkB kinase, Protein kinase B kinase, hPDK1, kinase PDK1

Accession NoSwiss-Prot: O15530
NCBI Protein: NP_002604.1

Uniprot O15530

Gene ID 5170;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 63kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from MCF, 293 and PC12 cells using PDK1(Ab-241) Antibody #21005.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PDK1(Ab-241) Antibody #21005(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF7 cells using PDK1(Ab-241) Antibody #21005.
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development

Scheid MP,et al. (2005)Mol Cell Biol; 25(6): 2347-63
Chen H, et al. (2001) Biochemistry; 40(39): 11851-9
Sato S,et al. (2002) J Biol Chem; 277(42): 39360-7
Lim MA, et al.(2003)Proc Natl Acad Sci U S A; 100(24): 14006-11
Beausoleil SA, et al. (2004)Proc Natl Acad Sci U S A; 101(33): 12130-5

If you have published an article using product 21005, please notify us so that we can cite your literature.

et al,PDK1-Foxo1 in Agouti-Related Peptide Neurons Regulates Energy Homeostasis by Modulating Food Intake and Energy Expenditure , (2011),
PMID: 21694754
et al,AXL phosphorylates and up-regulates TNS2 and its implications in IRS-1-associated metabolism in cancer cells.In J Biomed Sci On 2018 Nov 12 By Li-Chun Cheng, Yen-Lin Chen et al..PMID: 30419905 , (2018),
PMID: 30419905
et al,Activation of mammalian target of rapamycin pathway confers adverse outcome in nonsmall cell lung carcinoma锟?In Cancer on 2011 Aug 15 by Dan Liu, Yi Huang, et al..PMID:21387259 , (2011),
PMID: 21387259
et al,Phosphoproteome analysis reveals an important role for glycogen synthase kinase-3 in perfluorododecanoic acid-induced rat liver toxicity. In Toxicol Lett on 2013 Mar 27 by Hongxia Zhang, Junjie Hou, et al..PMID: 23353032 , (2013),
PMID: 23353032
et al,PDK1-Foxo1 in agouti-related peptide neurons regulates energy homeostasis by modulating food intake and energy expenditure. In PLoS One on 2011 by Yongheng Cao, Masanori Nakata, et al..PMID: 21694754 , (2011),
PMID: 21694754
et al,Notch-induced hIL-6 production facilitates the maintenance of self-renewal of hCD34+ cord blood cells through the activation of Jak-PI3K-STAT3 pathway. In Am J Pathol on 2012 Jan by Bongkum Choi, Eunyoung Chun, et al..PMID:22062221 , (2012),
PMID: 22062221

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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