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GSK3a(Ab-21) Antibody#21007

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Product Detail

Product NameGSK3a(Ab-21) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total GSK3a protein. and does not detect GSK-3b protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.19~23 (T-S-S-F-A) derived from Human GSK3a.

Target NameGSK3a

ConjugateUnconjugated

Other NamesFactor A, GSK-3 alpha, kinase GSK3-alpha,

Accession NoSwiss-Prot: P49840
NCBI Protein: NP_063937.2

Uniprot P49840

Gene ID 2931;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 51kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 cells using GSK3a(Ab-21) Antibody #21007.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3a(Ab-21) Antibody #21007(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF7 cells using GSK3a(Ab-21) Antibody #21007.
Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN

Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178.
Koivisto L, et al. (2003) J Cell Sci. 116(Pt 18): 3749-3760.
Welsh G I, et al. (1996) Trends Cell Biol. 6:274-279.
Srivastava A K, et al. (1998) Mol. Cell. Biochem. 182: 135-141.
Cross D A, et al. (1995) Nature. 378: 785-789.

If you have published an article using product 21007, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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