Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Location: Home > Polyclonal Antibodies > NFkB-p100(Ab-870) Antibody

NFkB-p100(Ab-870) Antibody#21016

Availability: Yes
Total Amount:$
Product Detail

Product NameNFkB-p100(Ab-870) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total NFkB-p100 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.868~872 (Y-G-S-Q-S) derived from Human NFkB-p100.

Target NameNFkB-p100

ConjugateUnconjugated

Other NamesDNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10; Lyt10; NFKB2

Accession NoSwiss-Prot: Q00653
NCBI Protein: NP_001070961.1

Uniprot Q00653

Gene ID 4791;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 120 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
 

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFkB-p100(Ab-870) Antibody #21016(left) or the same antibody preincubated with blocking peptide(right).
Western blot analysis of extracts from HL60 cells using NFkB-p100(Ab-870) Antibody #21016.

NFKB has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth.

Qu Z, et al. (2004) J Biol Chem; 279(43): 44563-72.
Xiao G, et al. (2001) J Biol Chem 7(2): 401-9.
 

If you have published an article using product 21016, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;