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JunD(Ab-255) Antibody#21028

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Product Detail

Product NameJunD(Ab-255) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total JunD protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 253~257 (G-E-S-P-P) derived from Human JunD.

Target NameJunD

ConjugateUnconjugated

Other NamesAP-1

Accession NoSwiss-Prot: P17535
NCBI Protein: NP_005345.3

Uniprot P17535

Gene ID 3727;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 38kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from HT29 and 293 cells using JunD(Ab-255) Antibody #21028.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunD(Ab-255) Antibody #21028
Immunofluorescence staining of methanol-fixed Hela cells using JunD(Ab-255) Antibody #21028.

Binds an AP-1 site and upon cotransfection stimulates the activity of a promoter that bears an AP-1 site.

Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101(33): 12130-12135.

If you have published an article using product 21028, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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