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Location： Home > Polyclonal Antibodies > STAT1(Ab-701) Antibody

STAT1(Ab-701) Antibody#21044

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Product Detail

Product NameSTAT1(Ab-701) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu

SpecificityThe antibody detects endogenous level of total STAT1 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 699~703 (T-G-Y-I-K) derived from Human STAT1.

Target NameSTAT1

ConjugateUnconjugated

Other NamesCANDF7, ISGF-3, STAT91

Accession NoSwiss-Prot: P42224
NCBI Protein: NP _009330.1

Uniprot P42224

Gene ID 6772;

Sdspage MW84,91kd

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 84,91kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from Hela, A431 and JK cells using STAT1(Ab-701) Antibody #21044.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT1(Ab-701) Antibody #21044(left) or the same antibody preincubated with blocking peptide(right).

Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.

Heim M H, (1999) J Recept Signal Transduct Res. 19: 75-120.
Durbin J E, et al. (1996) Cell. 84: 443-450.
Meraz M A, et al. (1996) Cell. 84: 431-442.
Wakao H, et al. (1994) EMBO J. 13: 2182-2191.
Demoulin J, B. et al. (1999) J Biol Chem. 274: 25855-258
Ihle J N, et al. (1994) Trends Biochem Sci. 19: 222-227.

If you have published an article using product 21044, please notify us so that we can cite your literature.

et al,A guanidine-rich regulatory oligodeoxy-nucleotideimproves type-2 diabetes in obese mice by blocking T-cell differentiation. , (2012),
PMID: 23027613
et al,Danzhi Jiangtang Capsule ameliorates kidney injury via inhibition of the JAK-STAT signaling pathway and increased antioxidant capacity in STZ-induced diabetic nephropathy rats.In Biosci Trends.On 2019 Jan 22 by Sun M, Bu W et al..PMID: 30606979 , (2018),
PMID: 30606979
et al,A thiazole-derived oridonIn analogue exhibits antitumor activity by directly and allosterically InhibitIng STAT3. In J Biol Chem on 2019 Nov 15;by Shen X, Zhao L,et al..PMID:31594861 , (2019),
PMID: 31594861
et al,A Guanidine-Rich Regulatory Oligodeoxynucleotide Improves type-2 Diabetes in Obese Mice by Blocking T-cell Differentiation. In EMBO Mol Med on 2012 Oct by Xiang Cheng, Jing Wang, et al..PMID:23027613 , (2012),
PMID: 23027613
et al,DJC suppresses Advanced Glycation End Products-Induced JAK-STAT signaling and ROS in mesangial cells.In Evid Based Complement Alternat Med on 2017 May 29 by Min Sun, Yan Li ,et al..PMID: 28630633 , (2017),
PMID: 28630633
et al,Expression of interferon effector gene SART1 correlates with interferon treatment response against hepatitis B infection. In Mediators Inflamm on 2016 by Yong Li, Chuanlong Zhu et al..PMID: 28077916 , (2016),
PMID: 28077916
et al,ycobacterium tuberculosis EspB protein suppresses interferon-纬-induced autophagy in murine macrophages.In J Microbiol Immunol Infect on 2016 Dec by DanDan Huang, Lang Bao et al..PMID:25641595 , (2016),
PMID: 25641595
More
et al,IL-17 intensifies IFN-纬-induced NOS2 upregulation in RAW 264.7 cells by further activating STAT1 and NF-魏B.In Int J Mol Med on 2016 byQiyue Gao, Yang Liu et al..PMID:26677135 , (2016),
PMID: 26677135
et al,Costunolide improved dextran sulfate sodium-induced acute ulcerative colitis in mice through NF-kB, STAT1/3, and Akt signaling pathways. In Int Immunopharmacol on 2020 Jul by Fan Xie, Hai Zhang, et al..PMID:32413737 , (2020),
PMID: 32413737

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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