Product Detail
Product NameCREB(Ab-133) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous level of total CREB protein and it also detects the CREB-related protein ATF-1.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa. 131~135 (R-P-S-Y-R) derived from Human CREB.
Target NameCREB
ConjugateUnconjugated
Other NamesCREB-1; CREB1;
Accession NoSwiss-Prot: P16220
NCBI Protein: NP _004370.1
Uniprot
P16220
Gene ID
1385;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 43kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293, HT29 and Hela cells using CREB(Ab-133) Antibody #21052.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB(Ab-133) Antibody #21052(left) or the same antibody preincubated with blocking peptide(right).
This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
Xing J, et al. (1998) Mol Cell Biol 18(4): 1946-55.
Tan Y, et al.( 1996) EMBO J; 15(17): 4629-42.
Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385.
Mayo LD, et al. (2001) Biol Chem; 276(27): 25184-9.
Lu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934.
If you have published an article using product 21052, please notify us so that we can cite your literature.
et al,Aluminum toxicity related to SOD and expression of presenilin and CREB in Bombyx mori.In Arch Insect Biochem Physiol.On 2018 Oct by Liu L, Qian X et al..PMID:29978503
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et al,Inhibition of TPL2 by interferon-α suppresses bladder cancer through activation of PDE4D.In J Exp Clin Cancer Res. On 2018 Nov 27 by Qiang Z, Zhou ZY et al..PMID: 30482227
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30482227
et al,Lithium chloride administration prevents spatial learning and memory impairment in repeated cerebral ischemia-reperfusion mice by depressing apoptosis and increasing BDNF expression in hippocampus.In Behav Brain Res.On 2015 Sep 15 by Fan M, Jin W et al..PMID:26031381
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et al,Downregulation of microsomal glutathione-S-transferase 1 modulates protective mechanisms in differentiated PC12 cells.In J Physiol Biochem on 2014 Jun by Monika Sobczak , Tomasz Boczek et al ..PMID: 24419913
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PMID:
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et al,Hydrogen sulfide decreases 尾-adrenergic agonist-stimulated lung liquid clearance by inhibiting ENaC-mediated transepithelial sodium absorption. In Am J Physiol Regul Integr Comp Physiol on 2015 Apr 1 by Alisa M Agn茅, Jan-Peter Baldin, et al..PMID: 25632025
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PMID:
25632025
et al,Connexin Hemichannels Contribute to the Activation of cAMP Signaling Pathway and Renin Production. In Int J Mol Sci on 2020 Jun 23 by Jingru Hong,
Jian Yao,et al..PMID:32585970
, (2020),
PMID:
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et al,Hydrogen sulfide decreases β-adrenergic agonist-stimulated lung liquid clearance by inhibiting ENaC-mediated transepithelial sodium absorption
, (2015),
PMID:
Yes
