Product Detail
Product NameAkt(Ab-308) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total Akt protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.306~310 (M-K-T-F-C) derived from Human AKT1.
Target NameAkt
ConjugateUnconjugated
Other Names RAC-PK-alpha; Protein kinase B;
Accession NoSwiss-Prot: P31749
NCBI Protein: NP_001014431.1
Uniprot
P31749
Gene ID
207;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
Tremblay F, et al. (2005) Diabetes; 54(9): 2674-84.
Xu BE, et al. (2005) J Biol Chem; 280(40): 34218-23.
Samuels Y, et al. (2005) Cancer Cell; 7(6): 561-73.
Di Maira G, et al. (2005) Cell Death Differ; 12(6): 668-77.
If you have published an article using product 21055, please notify us so that we can cite your literature.
et al,TRPV2 channel negatively controls glioma cell proliferation and resistance to Fas-induced apoptosis in ERK-dependent manner.
, (2010),
PMID:
20093382
et al,Anti inflammatory and metabolic reprogramming effects of MENK produce antitumor response in CT26 tumor bearing mice.In J Leukoc Biol on 2020 Jan 29. by Tuo Y, Zhang Z, et al..PMID:31994797
, (2020),
PMID:
31994797
et al,Combination of Ruthenium Complex and Doxorubicin Synergistically Inhibits Cancer Cell Growth by Down-Regulating PI3K/AKT Signaling Pathway.In Front Oncol on 2020 Feb 18 by Lin K, Rong Y, et al..PMID:32133289
, (2020),
PMID:
32133289
et al,Phytoestrogens protect joints in collagen induced arthritis by increasing IgG glycosylation and reducing osteoclast activation.In Int Immunopharmacol on 2020 Mar 12 by Du N, Song L, et al..PMID:32172207
, (2020),
PMID:
32172207
et al,MicroRNA-19a/b Regulates Multidrug Resistance in Human Gastric Cancer Cells by Targeting PTEN.In Biochem Biophys Res Commun on 2013 May 10 by Ting Li, Bin Zhang,et al..PMID: 23603256
, (2013),
PMID:
23603256
et al,TRPV2 channel negatively controls glioma cell proliferation and resistance to Fas-induced apoptosis in ERK-dependent manner.In
Carcinogenesis on 2010 May by Nabissi M, Morelli MB,et al..PMID:20093382
, (2010),
PMID:
20093382
et al,Fatty Acid Synthase Regulates Proliferation and Migration of Colorectal Cancer Cells via HER2-PI3K/Akt Signaling Pathway.In Nutr Cancer on 2012 Aug by Nan Li, Xiaodong Bu,et al..PMID:22860766
, (2012),
PMID:
22860766
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et al,Suppression of Hypoxia-Induced HIF-1alpha Accumulation by VEGFR Inhibitors: Different Profiles of AAL993 Versus SU5416 and KRN633.In Cancer Lett on 2010 Oct 1 by Hyun Seung Ban, Masaharu Uno,et al..PMID:20378243
, (2010),
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et al,Choline Prevents Fetal Overgrowth and Normalizes Placental Fatty Acid and Glucose Metabolism in a Mouse Model of Maternal Obesity.In J Nutr Biochem on 2017 Nov by Juha Nam , Esther Greenwald,et al..PMID: 28915389
, (2017),
PMID: 28915389
et al,A MicroRNA Signature in Gestational Diabetes Mellitus Associated With Risk of Macrosomia.In Cell Physiol Biochem on 2015 by Jiandong Li, Liping Song et al..PMID:26302821
, (2015),
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et al,In Vitro and In Vivo Evaluation of Antidiabetic Properties and Mechanisms of Ficus tikoua Bur.. In Nutrients on 2022 Oct 20 by Hanlei Wang, Kun Zhang,et al..PMID:36297098
, (2022),
PMID: 36297098
et al,Bioinformatics and validation reveal the potential target of curcumin in the treatment of diabetic peripheral neuropathy
, (2024),
PMID:
Yes
