Product Detail
Product Namep95/NBS1(Ab-343) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total p95/NBS1 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.341~345 (S-L-S-Q-G) derived from Human p95/NBS1.
Target Namep95/NBS1
ConjugateUnconjugated
Other NamesNBN
Accession NoSwiss-Prot: O60934
NCBI Protein: NP_002476.2
Uniprot
O60934
Gene ID
4683;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 95kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Jurkat cells using p95/NBS1(Ab-343) Antibody #21058 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p95/NBS1(Ab-343) Antibody #21058(left) or the same antibody preincubated with blocking peptide(right).
Mutations in p95/NBS1 gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.
Hsu HL, et al (2005)Oncogene; 24(31): 4956-64.
Falck J, et al. (2005) Nature; 434(7033): 605-11.
Buscemi G, et al. (2004) Oncogene; 23(46): 7691-700.
Beausoleil SA, et al. (2004) Proc Natl Acad Sci U S A; 101(33): 12130-5.
If you have published an article using product 21058, please notify us so that we can cite your literature.
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