Product Detail
Product NameBCL-2(Ab-56) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC IF
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total BCL-2 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa. 54~58 (G-H-T-P-H) derived from Human BCL-2.
Target NameBCL-2
ConjugateUnconjugated
Other NamesBCL2
Accession NoSwiss-Prot: P10415
NCBI Protein: NP_000624.2
Uniprot
P10415
Gene ID
596;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 26kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Hela, A2780 and 293 cells using BCL-2(Ab-56) Antibody #21059.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BCL-2(Ab-56) Antibody #21059(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using BCL-2(Ab-56) Antibody #21059.
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Ling, Y. H. et al. (1998) J. Biol. Chem. 273, 18984-18991.
Huang, S.J. and Cidlowski, J.A. (2002) FASEB 16, 825-832.
Deng, X. et al. (2001) J. Biol. Chem. 276, 23681-23688.
Huang ST,et al. (2002) FASEB J Jun; 16(8): 825-32.
Yamamoto, K. et al. (1999) Mol. Cell. Biol. 19, 8469-8478.
If you have published an article using product 21059, please notify us so that we can cite your literature.
et al,Lentivirus-mediated Sirt1 shRNA and resveratrol independently induce porcine preadipocyte apoptosis by canonical apoptotic pathway.
, (2013),
PMID:
23065251
et al,Anticancer effects of sodium butyrate on hepatocellular carcinoma cells in vitro. In Int J Mol Med on 2013 Apr by Hong-Gang Wang, Xiao-Dan Huang, et al..PMID:
23440283
, (2013),
PMID:
23440283
et al,miR-375 inhibits Helicobacter pylori-induced gastric carcinogenesis by blocking JAK2鈥揝TAT3 signaling.In Cancer Immunol Immunother on 2014 Jul by Lin Miao , Kunmei Liu et al..PMID:24718681
, (2014),
PMID:
24718681
Yes
