Product Detail
Product NameBAD(Ab-112) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total BAD protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.110~114 (H-S-S-Y-P) derived from Mouse BAD.
Target NameBAD
ConjugateUnconjugated
Other NamesBbc2
Accession NoSwiss-Prot: Q61337
NCBI Protein: NP_031548.1
Uniprot
Q61337
Gene ID
12015;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 23kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293 cells using BAD(Ab-112) Antibody #21062 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD(Ab-112) Antibody #21062(left) or the same antibody preincubated with blocking peptide(right).
The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
Zhang B, et al. (2004). Mol Cell Biol.24 (14): 6205-6214.
Rice PL, et al. (2003). Cancer Res.63 (3): 616-620.
Wang XQ, et al. (2001). J Biol Chem.276 (48): 44504-44511.
If you have published an article using product 21062, please notify us so that we can cite your literature.
et al,Xanthohumol exerts protective effects in liver alterations associated with agIng. In Eur J Nutr on 2019 Mar by Fern ndez-Garc a C, Rancan L, et al.. PMID: 29536163
, (2019),
PMID:
29536163
et al,Protective effect of resveratrol against inflammation, oxidative stress and apoptosis in pancreas of aged SAMP8 mice.In Exp Gerontol on 2017 Apr by Cristina Gin茅s , Sara Cuesta,,et al..PMID: 28130161
, (2017),
PMID:
28130161
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