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BAD(Ab-136) Antibody#21063

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Product NameBAD(Ab-136) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total BAD protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.134~138 (S-R-S-A-P) derived from Mouse BAD.

Target NameBAD

ConjugateUnconjugated

Other NamesBbc2

Accession NoSwiss-Prot: Q61337
NCBI Protein: NP_031548.1

Uniprot Q61337

Gene ID 12015;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 23kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from HT29 and HepG2 cells using BAD(Ab-136) Antibody #21063.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD(Ab-136) Antibody #21063(left) or the same antibody preincubated with blocking peptide(right).

The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

Wang XQ, et al. (2001). J Biol Chem.276 (48): 44504-44511.
Lee YI, et al. (2001). J Biol Chem.276 (20): 16969-16977.
Maiti D, et al. (2001). J Biol Chem.276 (1): 329-333.

If you have published an article using product 21063, please notify us so that we can cite your literature.

et al,PIWI-Interacting RNA-004800 Is Regulated by S1P Receptor Signaling Pathway to Keep Myeloma Cell Survival.In Front Oncol on 2020 Apr 15 by Ma H, Wang H, et al..PMID: 32351883 , (2020),
PMID: 32351883
et al,Anticancer effects of sodium butyrate on hepatocellular carcinoma cells in vitro. In Int J Mol Med on 2013 Apr by Hong-Gang Wang, Xiao-Dan Huang, et al..PMID: 23440283 , (2013),
PMID: 23440283
et al,miR-137 and miR-197 induce apoptosis and suppress tumorigenicity by targeting MCL-1 in multiple myeloma.In Clin Cancer Res on 2015 May 15 by Yijun Yang , Fei Li et al..PMID:25724519 , (2015),
PMID: 25724519
et al,Blocking the bFGF/STAT3 interaction through specific signaling pathways induces apoptosis in glioblastoma cells.In J Neurooncol 2014 Oct by Jingchao Wu , Xuequan Feng et al..PMID: 25048528 , (2014),
PMID: 25048528

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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