Product Detail
Product NameBAD(Ab-155) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total BAD protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.153~157 (R-M-S-D-E) derived from Mouse BAD.
Target NameBAD
ConjugateUnconjugated
Other NamesBbc2
Accession NoSwiss-Prot: Q61337
NCBI Protein: NP_031548.1
Uniprot
Q61337
Gene ID
12015;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 23kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293 cells using BAD(Ab-155) Antibody #21064 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD(Ab-155) Antibody #21064(left) or the same antibody preincubated with blocking peptide(right).
The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
Moon EY, et al. (2003). Blood.101 (10): 4122-4130.
If you have published an article using product 21064, please notify us so that we can cite your literature.
et al,Loss of Bad expression confers poor prognosis in non-small cell lung cancer
, (2012),
PMID:
21918885
et al,Loss of Bad expression confers poor prognosis in non-small cell lung cancer. In Med Oncol on 2012 Sep by Huang Y, Liu D,
et al..PMID:21918885
, (2012),
PMID:
21918885
Yes
