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HER2(Ab-877) Antibody#21070

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Product Detail

Product NameHER2(Ab-877) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total HER2 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.875~879 (T-E-Y-H-A) derived from Human HER2.

Target NameHER2

ConjugateUnconjugated

Other NamesC-erbB-2; ErbB2;

Accession NoSwiss-Prot: P04626
NCBI Protein: NP _001005862.1

Uniprot P04626

Gene ID 2064;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 185kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from SKOV3 cells using HER2(Ab-877) Antibody #21070.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HER2(Ab-877) Antibody #21070(left) or the same antibody preincubated with blocking peptide(right).

Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-a and amphiregulin.

Dittadi, R. et al. (2000) J. Natl. Cancer Inst. 92, 1443-1444.
Muthuswamy, S. K. et al. (1999) Mol. Cell. Biol. 19, 6845-6857.
Qian, X. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 1500-1504.
Kwon, Y. K. et al. (1997) J. Neurosci. 17, 8293-8299.
Klapper, L. N. et al. (2000) Cancer Res. 60, 3384-3388.

If you have published an article using product 21070, please notify us so that we can cite your literature.

et al,Fatty Acid Synthase Regulates Proliferation and Migration of Colorectal Cancer Cells via HER2-PI3K/Akt Signaling Pathway.In Nutr Cancer on 2012 Aug by Nan Li, Xiaodong Bu,et al..PMID:22860766 , (2012),
PMID: 22860766
et al,Loss of Fatty Acid Synthase Inhibits the "HER2-PI3K/Akt Axis" Activity and Malignant Phenotype of Caco-2 Cells.In Lipids Health Dis on 2013 Jun 1 by Nan Li, Heng Lu,et al..PMID:23725225 , (2013),
PMID: 23725225
et al,The 鈥淗ER2鈥揚I3K/Akt鈥揊ASN Axis锟?Regulated Malignant Phenotype of Colorectal Cancer Cells. In Lipids on 2012 Apr by Nan Li, Xiaodong Bu, et al..PMID:22218925 , (2012),
PMID: 22218925

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;