Product Detail
                                
                                                                        Product NameHER2(Ab-1248) Antibody
                                                                                 Host SpeciesRabbit
                                         ClonalityPolyclonal
                                                                                PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. 
                                        ApplicationsWB IHC IF
                                        Species ReactivityHu Ms Rt
                                        SpecificityThe antibody detects endogenous level of total HER2 protein.
                                        Immunogen TypePeptide-KLH
                                         Immunogen DescPeptide sequence around aa. 1246~1250 (P-E-Y-L-G) derived from Human HER2.
                                         Target NameHER2
                                         ConjugateUnconjugated
                                                                                 Other NamesC-erbB-2; ErbB2; 
                                        Accession NoSwiss-Prot: P04626
NCBI Protein: NP_001005862.1 
                                        Uniprot
                                        P04626
                                        
                                        Gene ID
                                        2064;
                                        
                                                                                                                        Concentration1.0mg/ml
                                         FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. 
                                        StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use. 
                                                                                                                                                         
                                Application Details
                                Predicted MW: 185kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
                             
                            
                                                                
                                    
                                    Western blot analysis of extracts from MCF and SKOV3 cells using HER2(Ab-1248) Antibody #21072.
                                 
                                                                
                                    
                                    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HER2(Ab-1248) Antibody #21072(left) or the same antibody preincubated with blocking peptide(right).
                                 
                                                                
                                    
                                    Immunofluorescence staining of methanol-fixed MCF7 cells using HER2(Ab-1248) Antibody #21072.
                                 
                                                             
                            
                                Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-a and amphiregulin.
Ramsauer VP, et al. (2003)J Biol Chem; 278(32): 30142-7.
Jepson S, et al. (2002)Oncogene; 21(49): 7524-32.
Ren Z, et al. (2002)J Biol Chem; 277(41): 38486-93.
Eppenberger-Castori S, et al. (2001)J Clin Oncol; 19(3): 645-56.
                            
                            
                                If you have published an article using product 21072, please notify us so that we can cite your literature.