Product Detail
Product NameEGFR(Ab-1092) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu Rt
SpecificityThe antibody detects endogenous level of total EGFR protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa. 1090~1094 (P-E-Y-I-N) derived from Human EGFR.
Target NameEGFR
ConjugateUnconjugated
Other Names Receptor tyrosine-protein kinase ErbB-1
Accession NoSwiss-Prot: P00533
NCBI Protein: NP_005219.2
Uniprot
P00533
Gene ID
1956;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 175kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from HepG2, 293 and Hela cells using EGFR(Ab-1092) Antibody #21074.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR(Ab-1092) Antibody #21074(left) or the same antibody preincubated with blocking peptide(right).
Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentia
Buerger C, et al. (2003) J Biol Chem; 278(39): 37610-21.
Wang XQ, (2003) J Biol Chem; 278(49): 48770-8.
Saito T, et al. (2004) Endocrinology; 145(9): 4232-43.
Pao W, et al. (2004) Proc Natl Acad Sci U S A; 101(36): 13306-11.
Mattila E, et al. (2005) Nat Cell Biol; 7(1): 78-85.
If you have published an article using product 21074, please notify us so that we can cite your literature.
et al,Cytoplasmic retention of a nucleocytoplasmic protein TBC1D3 by microtubule network is required for enhanced EGFR signaling.In PLoS One on 2014 Apr 8 by Ze He, Tian Tian et al..PMID:24714105
, (2014),
PMID:
24714105
Yes
