Product Detail
Product NameFAK(Ab-861) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total FAK protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa. 859~863 (H-I-Y-Q-P) derived from Human FAK.
Target NameFAK
ConjugateUnconjugated
Other NamesFADK 1; FAK1; PTK2
Accession NoSwiss-Prot: Q05397
NCBI Protein: NP _005598.3
Uniprot
Q05397
Gene ID
5747;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 125kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from HT29, Hela and 293 cells using FAK(Ab-861) Antibody #21076.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using FAK(Ab-861) Antibody #21076(left) or the same antibody preincubated with blocking peptide(right).
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Shi Q, et al. (2003) Mol Biol Cell; 14(10): 4306-15.
Vadlamudi RK, et al. (2003) FEBS Lett; 543(1-3): 76-80.
Eliceiri BP, et al. (2002) J Cell Biol Apr 01; 157(1): 149-60.
Abu-Ghazaleh R, (2001) et al. Biochem J; 360(Pt 1): 255-64.
Slack JK, et al.(2001) Oncogene; 20(10): 1152-63.
If you have published an article using product 21076, please notify us so that we can cite your literature.
et al,Effect of cell culture density on dental pulp-derived mesenchymal stem cells with reference to osteogenic differentiation. In Sci Rep on 2019 Apr 1 by Sonoko Noda, Nobuyuki Kawashima, et al..PMID: 30931957
, (2019),
PMID:
30931957
et al,Anticancer Effect of Heparin-Taurocholate Conjugate on Orthotopically Induced Exocrine and Endocrine Pancreatic Cancer
, (2021),
PMID:
34830928
Yes
