Product Detail
Product Namep53(Ab-18) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total p53 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa. 16~20 (Q-E-T-F-S) derived from Human p53.
Target Namep53
Other NamesTumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13
Accession NoSwiss-Prot: P04637
NCBI Protein: NP_000537.3
Uniprot
P04637
Gene ID
7157;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 53kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HT29 cells using p53(Ab-18) Antibody #21086.
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.
Lin T, et al. (2005) Nat Cell Biol; 7(2): 165-71.
Vega FM, et al. (2004) Mol Cell Biol; 24(23): 10366-80.
Li J, et al. (2004) J Biol Chem; 279(40): 41275-9.
Wang J, et al. (2004) J Biol Chem; 279(38): 39584-92.
If you have published an article using product 21086, please notify us so that we can cite your literature.
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