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Androgen Receptor(Ab-650) Antibody#21105

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Product Detail

Product NameAndrogen Receptor(Ab-650) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous level of total Androgen Receptor protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.648~652 (T-T-S-P-T) derived from Human Androgen Receptor.

Target NameAndrogen Receptor

ConjugateUnconjugated

Other NamesANDR; DHTR; AR

Accession NoSwiss-Prot: P10275
NCBI Protein: NP_000035.2

Uniprot P10275

Gene ID 367;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 110kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from Mouse kindey and brain tissue using Androgen Receptor (Ab-650) Antibody #21105.

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described.

Brinkman, A.O. et al. (1999) J. Steroid. Biochem. Mol. Biol. 69, 307-313.
Avila, D.M. et al. (2001) J. Steroid. Biochem. Mol. Biol. 76, 135-142.
Montgomery, J.S. et al. (2001) J. Pathol. 195, 138-146.

If you have published an article using product 21105, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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