Product Detail
Product NameRb(Ab-795) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total Rb protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.793~797 (P-S-S-P-L) derived from Human Rb.
Target NameRb
ConjugateUnconjugated
Other NamesP105-RB; PP105; PP110; RB-1; RB1
Accession NoSwiss-Prot: P06400
NCBI Protein: NP_000312.2
Uniprot
P06400
Gene ID
5925;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 110kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from MOLT cells using Rb(Ab-795) Antibody #21108 and the same antibody preincubated with blocking peptide.
Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Roesch A, et al. (2005) Mod Pathol. 18(4): 565-572.
Chadee DN, et al. (2004) Nat Cell Biol. 6(8): 770-776.
Knudsen ES, et al. (1997) Mol Cell Biol. 17(10): 5771-5783.
Knudsen ES, et al. (1996) J Biol Chem. 271(14): 8313-8320.
If you have published an article using product 21108, please notify us so that we can cite your literature.
et al, Nuclear distribution of claudin-2 increases cell proliferation in human lung adenocarcinoma cells.In Biochim Biophys Acta on 2014 Sep by Akira Ikari, Ryo Watanabe et al..PMID: 24907662
, (2014),
PMID:
24907662
Yes
