Product Detail
Product NameRb(Ab-807) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total Rb protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.805~809 (Y-I-S-P-L) derived from Human Rb.
Target NameRb
ConjugateUnconjugated
Other NamesP105-RB; PP105; PP110; RB-1; RB1
Accession NoSwiss-Prot: P06400
NCBI Protein: NP_000312.2
Uniprot
P06400
Gene ID
5925;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 110kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from K562 cells using Rb(Ab-807) Antibody #21109.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb(Ab-807) Antibody #21109(left) or the same antibody preincubated with blocking peptide(right).
Rb operates in the midst of the cell cycle clock apparatus. Its main role is to act as a signal transducer connecting the cell cycle clock with the transcriptional machinery (Ref.1). It plays an important role in the Rb/ E2F pathway in cell proliferation, cell fate determination, and cancer (Ref.2). Cellular senescence is a stable form of cell cycle arrest that limits proliferation of damaged cells and act as a natural barrier to cancer progression. A distinct heterochromatic structure that accumulates in senescent human fibroblasts, designated as SAHF (Senescence-Associated Heterochromatic Foci). SAHF formation coincides with recruitment of heterochromatin proteins and the Rb protein to E2F-responsive promoters and is associated with the stable repression of E2F target genes. Both SAHF formation and the silencing of E2F target genes depended on the integrity of the Rb pathway and do not occur in reversibly arrested cells (Ref.3). Rb activates transcription of the c-Jun gene through the SP1-binding site within the c-Jun promoter
Roesch A, et al. (2005) Mod Pathol. 18(4): 565-572.
Chadee DN, et al. (2004) Nat Cell Biol. 6(8): 770-776.
Knudsen ES, et al. (1997) Mol Cell Biol. 17(10): 5771-5783.
Knudsen ES, et al. (1996) J Biol Chem. 271(14): 8313-8320.
If you have published an article using product 21109, please notify us so that we can cite your literature.
et al,MS4a4B, a CD20 Homologue in T Cells, Inhibits T Cell Propagation by Modulation of Cell Cycle.
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Cai X, Ye T,et al..PMID:21601631
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et al,Vinpocetine Inhibits Breast Cancer Cells Growth in Vitro and in Vivo.In Apoptosis on 2012 Oct by Er-Wen Huang, Sheng-Jiang Xue, et al..PMID:22729609
, (2012),
PMID:
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et al,MS4a4B, a CD20 homologue in T cells, inhibits T cell propagation by modulation of cell cycle. In PLoS One on 2010 Nov 1 by Hui Xu, Yaping Yan, et al..PMID:21072172
, (2010),
PMID:
21072172
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