Product Detail
Product NameRb(Ab-780) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of taotal Rb protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa. 778~782 (T-L-S-P-I) derived from Human Rb.
Target NameRb
ConjugateUnconjugated
Other NamespRb
Accession NoSwiss-Prot: P06400
NCBI Protein: NP_000312.2
Uniprot
P06400
Gene ID
5925;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 110kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from JK cells using Rb(Ab-780) Antibody #21110.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb(Ab-780) Antibody #21110(left) or the same antibody preincubated with blocking peptide(right).
Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Roesch A, et al.(2005) Mod Pathol. 18(4): 565-572.
Huang H, et al.(2004) Oncogene. 23(12): 2161-2176.
Kitagawa M, et al.(1996)EMBO J. 15(24): 7060-7069.
If you have published an article using product 21110, please notify us so that we can cite your literature.
et al,The reversal effect of Ginsenoside Rh2 on drug resistance in human colorectal carcinoma cells and its mechanism. In Hum Cell.On 2018 Jul by Liu GW, Liu YH et al..PMID:29582366
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et al,T-helper-1-cell Cytokines Drive Cancer Into Senescence. In Nature on 2013 Feb 21 by Heidi Braum眉ller, Thomas Wieder, et al..PMID: 23376950
, (2013),
PMID:
23376950
et al,EEN regulates the proliferation and survival of multiple myeloma cells by potentiating IGF-1 secretion.In Biochem Biophys Res Commun on 2014 May 2 by Er-Wen Huang , Sheng-Jiang Xue et al ..PMID: 24704450
, (2014),
PMID:
24704450
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