Product Detail
Product NameCaveolin-1(Ab-14) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total Caveolin-1 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.12~16 (H-L-Y-T-V) derived from Human CAVEOLIN-1.
Target NameCaveolin-1
ConjugateUnconjugated
Other NamesCAV; CAV1;
Accession NoSwiss-Prot: Q03135
NCBI Protein: NP_001166366.1
Uniprot
Q03135
Gene ID
857;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 24kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from HT29 and HUVEC cells using Caveolin-1(Ab-14) Antibody #21112.
The scaffolding protein encoded by Caveolin-1 is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (a and beta) are encoded by a single transcript from this gene.
Zhang Y, et al. (2005) Mol Cell Proteomics. 4(9): 1240-1250.
Labrecque L, et al. (2004) J Biol Chem. 279(50): 52132-52140.
Fielding PE, et al. (2004) Biochemistry. 43(9): 2578-2586.
Labrecque L, et al. (2003) Mol Biol Cell. 14(1): 334-347.
Maggi D, et al. (2002) Biochem Biophys Res Commun. 295(5): 1085-1089.
If you have published an article using product 21112, please notify us so that we can cite your literature.
et al,Systematic analysis of mRNA expression profiles in NSCLC cell lines to screen metastasis-related genes.In Mol Med Rep.On 2016 Dec by Liu Y, Liu L et al..PMID:27840927
, (2016),
PMID:
27840927
Yes
