Product Detail
Product NameChk1(Ab-317) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total Chk1 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.315~319 (S-S-S-Q-P) derived from Human Chk1.
Target NameChk1
ConjugateUnconjugated
Other NamesCHEK1
Accession NoSwiss-Prot: O14757
NCBI Protein: NP_001107593.1
Uniprot
O14757
Gene ID
1111;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 56kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Chek1 is a protein kinase that inhibits mitotic entry after DNA damage, required for the DNA damage checkpoint and is strongly similar to murine Chek1. Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The mouse and human proteins share 90% sequence identity through the protein kinase domains. The sequence of the 476-amino acid human Chek1 protein is 29%, 40%, and 44% identical to those of the fission yeast Chek1, C. elegans Chek1, and Drosophila 'grapes' (Grp) proteins, respectively. Chek1 is expressed ubiquitously as an approximately 2.4-kb mRNA, with the most abundant expression in thymus, testis, small intestine, and colon. The protein has altered mobility when isolated from cells treated with ionizing radiation, indicating that Chek1 is modified in response to DNA damage. In vitro, Chek1 directly phosphorylates a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, Chek1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-Cyclin-B complex and mitotic entry
Zhang YW, et al. (2005) Mol Cell; 19(5): 607-18.
Bhoumik A, et al. (2005) Mol Cell; 18(5): 577-87.
Rocha S, et al. (2005) EMBO J.
Clarke CA, et al. (2005) Biochem J.
Yu X, et al. (2004) Mol Cell Biol; 24(21): 9478-86.
Yes
