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MKK3(Ab-189) Antibody#21116

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Product Detail

Product NameMKK3(Ab-189) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms

SpecificityThe antibody detects endogenous level of total MKK3 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.187~191 (V-D-S-V-A) derived from Human MKK3.

Target NameMKK3

ConjugateUnconjugated

Other NamesMAP kinase kinase 3; MAP2K3; MAPK/ERK kinase 3; MAPKK 3; MEK3

Accession NoSwiss-Prot: P46734
NCBI Protein: NP_002747.2

Uniprot P46734

Gene ID 5606;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 40kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from MDA cells using MKK3(Ab-189) Antibody #21116.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3(Ab-189) Antibody #21116(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using MKK3(Ab-189) Antibody #21116.

MEK3 belongs to MAPKK family. This kinase is activated by mitogenic and environmental stress, and participates in the MAPK-mediated signaling cascade. It phosphorylates and thus activates p38. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of Ras oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of p38, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis.

Wang W, et al. (2002) Mol Cell Biol ; 22(10): 3389-403.
Raingeaud J, et al. (1996) Mol Cell Biol; 16(3): 1247-55.

If you have published an article using product 21116, please notify us so that we can cite your literature.

et al,Suppression of phospho-p85α?CGTP-Rac1 lipid raft interaction by bichalcone analog attenuates cancer cell invasion. In Mol Carcinog on 2016 Dec by Hui-Li Lu, Shih-Shun Chen, et al..PMID: 26756739 , (2016),
PMID: 26756739

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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