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Chk2(Ab-516) Antibody#21117

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Product Detail

Product NameChk2(Ab-516) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB

Species ReactivityHu

SpecificityThe antibody detects endogenous levels of total Chk2 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 514~518(Q-P-S-T-S) derived from Human Chk2.

Target NameChk2

ConjugateUnconjugated

Other NamesCHEK2; RAD53;

Accession NoSwiss-Prot: O96017
NCBI Protein: NP_001005735.1

Uniprot O96017

Gene ID 11200;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 62kd
Western blotting: 1:500~1:1000

Western blot analysis of extract from HL60 cells using Chk2(Ab-516) Antibody #21117.

The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation

Melchionna, R. et al. (2000) Nat. Cell Biol. 2, 762-765.
Ahn, J.Y. et al. (2000) Cancer Res. 60, 5934-5936.
Lee, C.H. and Chung, J.H. (2001) J. Biol. Chem. 276, 30537-30541

If you have published an article using product 21117, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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