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JAK1(Ab-1022) Antibody#21119

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Product Detail

Product NameJAK1(Ab-1022) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total JAK1 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 1020~1024 (K-E-Y-Y-T) derived from Human JAK1.

Target NameJAK1

ConjugateUnconjugated

Other Names Janus kinase 1

Accession NoSwiss-Prot: P23458
NCBI Protein: NP_002218.2

Uniprot P23458

Gene ID 3716;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 130kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 3T3, HepG2, HT29 and MDA cells using JAK1(Ab-1022) Antibody #21119.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JAK1(Ab-1022) Antibody #21119(left) or the same antibody preincubated with blocking peptide(right).
Tyrosine kinase of the non-receptor type, involved in the IFN-a/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.

Zheng H, et al. (2005) Mol Cell Proteomics. 4(6):721-730.
Wang R, et al. (2003) Arch Biochem Biophys. 410(1): 7-15.

If you have published an article using product 21119, please notify us so that we can cite your literature.

et al,A thiazole-derived oridonIn analogue exhibits antitumor activity by directly and allosterically InhibitIng STAT3. In J Biol Chem on 2019 Nov 15;by Shen X, Zhao L,et al..PMID:31594861 , (2019),
PMID: 31594861
et al,Down-regulation of JAK1 by RNA interference inhibits growth of the lung cancer cell line A549 and interferes with the PI3K/mTOR pathway. In J Cancer Res Clin Oncol on 2011 Nov by Dan Liu, Yi Huang, et al..PMID:21861134 , (2011),
PMID: 21861134

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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