Product Detail
Product NameIkB-a(Ab-32/36) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC IF
Species ReactivityHu Ms
SpecificityThe antibody detects endogenous level of total IkB-a protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.30~34/34~38 (H-D-S-G-L- D-S -M-K) derived from Human IkB-a.
Target NameIkB-a
ConjugateUnconjugated
Other NamesI-kappa-B-alpha; IKBA; NF-kappaB inhibitor alpha; NFKBI; NFKBIA
Accession NoSwiss-Prot: P25963
NCBI Protein: NP_065390.1
Uniprot
P25963
Gene ID
4792;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 39kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 and 3T3 cells using IkB-a(Ab-32/36) Antibody #21122.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-a(Ab-32/36) Antibody #21122(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using IkB-a(Ab-32/36) Antibody #21122.
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.
Mattioli I, et al. (2004)J Immunol; 172(10): 6336-44.
Courtois G, et al. (2003)J Clin Invest; 112(7): 1108-15.
Nair A, et al. (2003) Oncogene; 22(1): 50-8.
Fan C, et al. (2002)J Cell Sci; 115(Pt 24): 4843-53.
Schubert SY, et al. (2002)FASEB J; 16(14): 1931-3.
If you have published an article using product 21122, please notify us so that we can cite your literature.
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