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IKK a(Ab-23) Antibody#21123

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Product Detail

Product NameIKK a(Ab-23) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total IKKa protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.21~25 (L-G-T-G-G) derived from Human IKK a.

Target NameIKK a

ConjugateUnconjugated

Other NamesI kappa-B kinase alpha; I-kappa-B kinase 1; IKK-A; IKK-alpha; IKK1

Accession NoSwiss-Prot: O15111
NCBI Protein: NP_001269.3

Uniprot O15111

Gene ID 1147;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 85kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela, 293 and 3T3 cells using IKK a(Ab-23) Antibody #21123.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using IKK a(Ab-23) Antibody #21123(left) or the same antibody preincubated with blocking peptide(right).
Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.

Yuan ZQ, et al.(2002)J Biol Chem; 277(33): 29973-82.
Ozes ON, et al. (1999)Nature; 401(6748): 82-5.

If you have published an article using product 21123, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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