Product Detail
                                
                                                                        Product NameASK1(Ab-83) Antibody
                                                                                 Host SpeciesRabbit
                                         ClonalityPolyclonal
                                                                                PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. 
                                        ApplicationsWB IHC IF
                                        Species ReactivityHu
                                        SpecificityThe antibody detects endogenous level of total ASK1 protein.
                                        Immunogen TypePeptide-KLH
                                         Immunogen DescPeptide sequence around aa.81~85 (G-S-S-V-G) derived from Human ASK1.
                                         Target NameASK1
                                         ConjugateUnconjugated
                                                                                 Other NamesASK-1; M3K5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5
                                        Accession NoSwiss-Prot: Q99683
NCBI Protein: NP_005914.1 
                                        Uniprot
                                        Q99683
                                        
                                        Gene ID
                                        4217;
                                        
                                                                                                                        Concentration1.0mg/ml
                                         FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. 
                                        StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use. 
                                                                                                                                                         
                                Application Details
                                Predicted MW: 155kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
                             
                            
                                                                
                                    
                                    Western blot analysis of extracts from MDA-MB- 435 cells using ASK1(Ab-83) Antibody #21125 and the same antibody preincubated with blocking peptide.
                                 
                                                                
                                    
                                    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1(Ab-83) Antibody #21125(left) or the same antibody preincubated with blocking peptide(right).
                                 
                                                                
                                    
                                    Immunofluorescence staining of methanol-fixed Hela cells using ASK1(Ab-83) Antibody #21125.
                                 
                                                             
                            
                                Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
Mabuchi S, et al. (2004) Endocrinology. 145(1): 49-58.
Yuan ZQ, et al. (2003) J Biol Chem. 278(26): 23432-23440.
Kim AH, et al. (2001) Mol Cell Biol. 21(3): 893-901. 
                            
                            
                                If you have published an article using product 21125, please notify us so that we can cite your literature.
                                                                                                    
                                      et al,ASK1鈥揓NK signaling cascade mediates Ad鈥怱T13鈥恑nduced apoptosis in colorectal HCT116 cells. In J Cell Biochem on 2010 Jun 1 by Min Yang, Mingcan Yu, et al..PMID:20512919 
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PMID:
                                    20512919