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ASK1(Ab-83) Antibody#21125

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Product Detail

Product NameASK1(Ab-83) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu

SpecificityThe antibody detects endogenous level of total ASK1 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.81~85 (G-S-S-V-G) derived from Human ASK1.

Target NameASK1

ConjugateUnconjugated

Other NamesASK-1; M3K5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5

Accession NoSwiss-Prot: Q99683
NCBI Protein: NP_005914.1

Uniprot Q99683

Gene ID 4217;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 155kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from MDA-MB- 435 cells using ASK1(Ab-83) Antibody #21125 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1(Ab-83) Antibody #21125(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using ASK1(Ab-83) Antibody #21125.
Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.

Mabuchi S, et al. (2004) Endocrinology. 145(1): 49-58.
Yuan ZQ, et al. (2003) J Biol Chem. 278(26): 23432-23440.
Kim AH, et al. (2001) Mol Cell Biol. 21(3): 893-901.

If you have published an article using product 21125, please notify us so that we can cite your literature.

et al,ASK1鈥揓NK signaling cascade mediates Ad鈥怱T13鈥恑nduced apoptosis in colorectal HCT116 cells. In J Cell Biochem on 2010 Jun 1 by Min Yang, Mingcan Yu, et al..PMID:20512919 , (),
PMID: 20512919

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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