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AMPKα1 (Ab-496)Antibody#21130

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Product Detail

Product NameAMPKα1 (Ab-496)Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu

SpecificityThe antibody detects endogenous level of total AMPKa1 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.494~498 (S-G-S-V-S) derived from Human AMPKα1.

Target NameAMPKa1

ConjugateUnconjugated

Other Names AAPK1; AMPK alpha-1 chain; AMPK-alpha1; HMG-CoA redustase kinase; PRKAA1

Accession NoSwiss-Prot:Q13131
Gene ID:5562

Uniprot Q13131

Gene ID 5562;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 63kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from 293 cells using AMPKα1 (Ab-496)Antibody #21130.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPKα1 (Ab-496)Antibody #21130 (left) or the same antibody preincubated with blocking peptide (right).

Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Kim JE, et al. (2005) J Proteome Res. 4(4): 1339-1346.
Woods A, et al. (2003) J Biol Chem. 278(31): 28434-28442.

If you have published an article using product 21130, please notify us so that we can cite your literature.

et al,Construction of adiponectin-encoding plasmid DNA and overexpression in mice in vivo.In Gene on 2012 Jul 10 by Huang YN, Qi JH, et al..PMID:22561699 , (2012),
PMID: 22561699

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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