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ASK1(Ab-966) Antibody#21134

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Product Detail

Product NameASK1(Ab-966) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total ASK1 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.964~968 (S-I-S-L-P) derived from Human ASK1.

Target NameASK1

ConjugateUnconjugated

Other NamesASK-1; M3K5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5

Accession NoSwiss-Prot: Q99683
NCBI Protein: NP_005914.1

Uniprot Q99683

Gene ID 4217;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 155kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from 293 and Hela cells using ASK1(Ab-966) Antibody #21134.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1(Ab-966) Antibody #21134(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using ASK1(Ab-966) Antibody #21134.

Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.

Zhang W, et al. (2005) J Biol Chem. 280(19): 19036-19044.
Fujii K, et al. (2004) Oncogene. 23(29):5099-5104.
Goldman EH, et al. (2004) J Biol Chem 2004 Mar 12; 279(11): 10442-10449.
Zhang L, et al. (1999) Proc Natl Acad Sci U S A. 96(15): 8511-8515.

If you have published an article using product 21134, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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