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HDAC4(Ab-632) Antibody#21141

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Product Detail

Product NameHDAC4(Ab-632) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IF

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total HDAC4 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa. 630~634 (A-Q-S-S-P) derived from Human HDAC4.

Target NameHDAC4

ConjugateUnconjugated

Other NamesHD4; Histone deacetylase 4;

Accession NoSwiss-Prot: P56524
NCBI Protein: NP_006028.2

Uniprot P56524

Gene ID 9759;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 140kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from HepG2 cells using HDAC4(Ab-632) Antibody #21141.
Immunofluorescence staining of methanol-fixed Hela cells using HDAC4(Ab-632) Antibody #21141.
Western blotting analysis using HDAC4(Ab-632) Antibody #21141.

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.

Wang AH, et al. (2000) Mol Cell Biol. 20(18): 6904-6912.
Grozinger CM, et al. (2000) Proc Natl Acad Sci U S A. 97(14): 7835-7840.

If you have published an article using product 21141, please notify us so that we can cite your literature.

et al,Anticancer effects of sodium butyrate on hepatocellular carcinoma cells in vitro. In Int J Mol Med on 2013 Apr by Hong-Gang Wang, Xiao-Dan Huang, et al..PMID: 23440283 , (2013),
PMID: 23440283
et al,Expression and localization of calmodulin-related proteins in brain, heart and kidney from spontaneously hypertensive rats.In Biochem Biophys Res Commun on 2016 Jan 15 by Satoshi Kameshima, Muneyoshi Okada et al..PMID:26697749 , (2016),
PMID: 26697749
et al,Thrombospondin-1 might be a therapeutic target to suppress RB cells by regulating the DNA double-strand breaks repair. In Oncotarget on 2016 Feb 2 by Pei Chen, Na Yu, et al..PMID:26756218 , (2016),
PMID: 26756218

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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