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HDAC5(Ab-498) Antibody#21142

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Product Detail

Product NameHDAC5(Ab-498) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total HDAC5 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.496~500 (T-Q-S-S-P) derived from Human HDAC5/7.

Target NameHDAC5

ConjugateUnconjugated

Other NamesHD5

Accession NoSwiss-Prot: Q9UQL6
NCBI Protein: NP_001015053.1

Uniprot Q9UQL6

Gene ID 10014;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 124kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from 293 and HepG2 cells using HDAC5(Ab-498) Antibody #21142.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC5(Ab-498) Antibody #21142(left) or the same antibody preincubated with blocking peptide(right).

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC5 belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.

Doppler H, et al. (2005) J Biol Chem. 280(15):15013-15019.
McKinsey TA, et al. (2000) Nature. 408(6808): 106-111.

If you have published an article using product 21142, please notify us so that we can cite your literature.

et al,GIT1 mediates HDAC5 activation by angiotensin II in vascular smooth muscle cells. , (2008),
PMID: 18292392
et al,Fluid shear stress stimulates phosphorylation-dependent nuclear export of HDAC5 and mediates expression of KLF2 and eNOS.In Blood on 2010 Apr 8 by Weiye Wang, Chang Hoon Ha,et al..PMID:20042720 , (2010),
PMID: 20042720

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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