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HSP27(Ab-15) Antibody#21144

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Product Detail

Product NameHSP27(Ab-15) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu

SpecificityThe antibody detects endogenous level of total HSP27 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.13~17 (G-P-S-W-D) derived from Human HSP27.

Target NameHSP27

ConjugateUnconjugated

Other Names28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Growth-related 25 kDa protein; HSP 27; HSP25

Accession NoSwiss-Prot: P04792
NCBI Protein: NP _001531.1

Uniprot P04792

Gene ID 3315;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details
Predicted MW: 27kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela cells using HSP27(Ab-15) Antibody #21144 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP27(Ab-15) Antibody #21144(left) or the same antibody preincubated with blocking peptide(right).
Involved in stress resistance and actin organization.

If you have published an article using product 21144, please notify us so that we can cite your literature.

et al,Upregulation of heat shock protein 27 confers resistance to actinomycin D鈥恑nduced apoptosis in cancer cells. In FEBS J on 2013 Sep by Wenbo Ma, Yan Teng,et al..PMID: 23848600 , (2013),
PMID: 23848600
et al,Anaplastic astrocytomas with abundant R osenthal fibers in elderly patients: A diagnostic pitfall of high鈥恎rade gliomas. In Neuropathology on 2013 Oct by Yasuo Sugita, Shinji Nakashima, et al..PMID: 23438297 , (2013),
PMID: 23438297

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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