Product Detail
Product NameFAK(Ab-925) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB IHC IF
Species ReactivityHu Ms Rt
SpecificityThe antibody detects endogenous level of total FAK protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.923~927 (K-V-Y-E-N) derived from Human FAK.
Target NameFAK
ConjugateUnconjugated
Other NamesFADK 1; FAK1; PTK2
Accession NoSwiss-Prot: Q05397
NCBI Protein: NP_005598.3
Uniprot
Q05397
Gene ID
5747;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 125kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Hela cells using FAK(Ab-925) Antibody #21148 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FAK(Ab-925) Antibody #21148(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using FAK(Ab-925) Antibody #21148.
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Sanders MA, et al. (2005) J Biol Chem; 280(25): 23516-22.
Cherubini A, et al. (2005) Mol Biol Cell; 16(6): 2972-83.
Toriumi Y, et al. (2003) FEBS Lett; 553(3): 419-22.
Shi Q, et al. (2003) Mol Biol Cell; 14(10): 4306-15.
If you have published an article using product 21148, please notify us so that we can cite your literature.
et al,PTBP3 contributes to the metastasis of gastric cancer by mediating CAV1 alternative splicing.In Cell Death Dis.On 2018 May 1 by PMID: 29752441
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et al,FOXQ1 Promotes Cancer Metastasis by PI3K/AKT Signaling Regulation in Colorectal Carcinoma.In Am J Transl Res on 2017 May 15 by Jia Yun Liu, Xiao Yu Wu,et al..PMID: 28559972
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et al,RGD and (−)-epigallocatechin-3-gallate modification enhanced biostability, bioactive and biofunctionality of acellular porcine cornea stroma crosslinked with oxidized dextran
, (2024),
PMID:
et al,Anticancer Effect of Heparin-Taurocholate Conjugate on Orthotopically Induced Exocrine and Endocrine Pancreatic Cancer
, (2021),
PMID:
34830928
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