Product Detail
Product Namep21Cip1(Ab-145) Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
ApplicationsWB
Species ReactivityHu
SpecificityThe antibody detects endogenous level of total p21Cip1 protein.
Immunogen TypePeptide-KLH
Immunogen DescPeptide sequence around aa.143~147 (R-Q-T-S-M) derived from Human p21Cip1.
Target Namep21Cip1
ConjugateUnconjugated
Other NamesCAP20; CDK-interacting protein 1; CDKN1; CDKN1A; CDN1A
Accession NoSwiss-Prot: P38936
NCBI Protein: NP _000380.1
Uniprot
P38936
Gene ID
1026;
Concentration1.0mg/ml
FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Application Details
Predicted MW: 21kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from Hela cells using p21Cip1(Ab-145) Antibody #21149 and the same antibody preincubated with blocking peptide.
May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.
Ocker M Int,et al. (2007)J Biochem Cell Biol. 39(7-8):1367-74.
Dangi S, et al.(2006)Cell Prolif. 39(4):261-79.
Chen J,et al.(2006)Am J Physiol Heart Circ Physiol. 290(4):H1575-86
If you have published an article using product 21149, please notify us so that we can cite your literature.
Yes
