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p27Kip1(Ab-10) Antibody#21150

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Product Detail

Product Namep27Kip1(Ab-10) Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

ApplicationsWB IHC

Species ReactivityHu Ms Rt

SpecificityThe antibody detects endogenous level of total p27Kip1 protein.

Immunogen TypePeptide-KLH

Immunogen DescPeptide sequence around aa.8~12 (N-G-S-P-S) derived from Human p27Kip1.

Target Namep27Kip1

ConjugateUnconjugated

Other NamesCDKN1B; CDN1B; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; KIP1

Accession NoSwiss-Prot: P46527
NCBI Protein: NP_004055.1

Uniprot P46527

Gene ID 1027;

Concentration1.0mg/ml

FormulationSupplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 27kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from A2780 cells using p27Kip1(Ab-10) Antibody
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using p27Kip1(Ab-10) Antibody

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.

Le XF, et al. (2003)J Biol Chem.
Connor MK, et al. (2003)Mol Biol Cell; 14(1): 201-13.
McAllister SS, et al. (2003) Mol Cell Biol; 23(1): 216-28.
Chopra S, et al. (2002)J Biol Chem; 277(36): 32413-6.
Boehm M, et al. (2002)EMBO J; 21(13): 3390-401.

If you have published an article using product 21150, please notify us so that we can cite your literature.

et al,Cloning and Characterization of a Novel Intracellular Protein p48.2 that Negatively Regulates Cell Cycle Progression. , (2009),
PMID: 19427400

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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